Fig. 1From: Development of diagnostic tools for IBDV detection using plants as bioreactorsExpression, semipurification, and quantification of SVP. Aliquots of the subsequent steps of the purification of the samples were loaded into 12% SDS-PAGE for a immunoblotting and b Coomassie blue staining. VP2 were identified using a rabbit anti-VP2 antibody diluted 1:500. The negative control consisted of leaves infiltrated with GFP subjected to the same purification process (see “Materials and methods”). c Standard curve of BSA—250, 125, 62.5, 31.25, 15.6 and 7.8 µg/mL—and VP2 were stained with Coomassie blue reagent and the intensity of the bands were analyzed with Gel-Pro Analyzer software v3.1. d Transmission electron micrographs of SVP. The dialysate was subjected to negative staining with 2% phosphotungstic acid and observed under an electronic microscope (CM 200, PHILIPS). Scale bar of large pictures = 200 nmBack to article page