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Fig. 1 | AMB Express

Fig. 1

From: Development of diagnostic tools for IBDV detection using plants as bioreactors

Fig. 1

Expression, semipurification, and quantification of SVP. Aliquots of the subsequent steps of the purification of the samples were loaded into 12% SDS-PAGE for a immunoblotting and b Coomassie blue staining. VP2 were identified using a rabbit anti-VP2 antibody diluted 1:500. The negative control consisted of leaves infiltrated with GFP subjected to the same purification process (see “Materials and methods”). c Standard curve of BSA—250, 125, 62.5, 31.25, 15.6 and 7.8 µg/mL—and VP2 were stained with Coomassie blue reagent and the intensity of the bands were analyzed with Gel-Pro Analyzer software v3.1. d Transmission electron micrographs of SVP. The dialysate was subjected to negative staining with 2% phosphotungstic acid and observed under an electronic microscope (CM 200, PHILIPS). Scale bar of large pictures = 200 nm

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