Rich production media as a platform for CHO cell line development

AMB Express

Table 2 Evaluation of media performance based on platform media

Media candidates^b	Relative productivity for IgG^d	Relative productivity for recombinant protein^d	Cell aggregation^d	Total amino acid content range (excluding glutamine)^a
CDM1^c (CDM4CHO)	Moderate	Moderate	Negligible	2.0–3.0
CDM2 (HyCell CHO)	High	High	Negligible	4.5–5.5
CDM3 (ActiPro)	High	Moderate	Negligible	6.5–7.5
CD FortiCHO	High	High	Usually observed	7.5–9.0
Cellvento CHO-210	Low	High	Frequently observed	3.5–4.5
ExCell Advanced CHO	Moderate	Low	Negligible	7.0–8.0
PowerCHO-2	Moderate	Poor	Usually observed	10.5–12.5

^aExpected ranges are from internal amino acid analysis and/or references (Reinhart et al. 2015; Carrillo-Cocom et al. 2015; Pan et al. 2017). Amino acids are detected (and reported in publications) as molar mass, so the amino acid concentration is calculated with molecular weight
^bCDM4CHO (GE Healthcare, Björkgatan, Uppsala, Sweden), HyCell CHO (GE Healthcare, Björkgatan, Uppsala, Sweden), ActiPro (GE Healthcare, Björkgatan, Uppsala, Sweden), CD FortiCHO (Thermo Fisher Scientific, Waltham, MA, US), Cellvento CHO-210 (Merck KGaA, Darmstadt, Germany), ExCell Advanced CHO (SAFC Bioscience, St, Louis, Mo, USA), PowerCHO 2 (Lonza, Verviers, Belgium)
^cCDM1 is an earlier commercial CHO media. The rest are recent commercial CHO media according to our classification
^dThe cell lines tested here are developed from our CLD platform using CDM1, the results of each media here may be different in other cell lines