Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds

AMB Express

Table 6 Showed all data (PEAKS 8.5 software) of the complete amino acid sequences of fava bean lectin (Favin P02871|LEC_VICFA)

Peptide sequences (this study)	− 10lgP	Mass	Length	ppm	m/z	RT	Scan	Intensity sample	Peptide location		PTM
Peptide sequences (this study)	− 10lgP	Mass	Length	ppm	m/z	RT	Scan	Intensity sample	Start	End	PTM
TDEITSFSIPK.F	45.59	1236.62	11	3.2	619.32	18.45	2968	3.28E4	1	11
K.FRPDQPNLIFQGGGYTTK.E	39.35	2038.03	18	3.5	680.35	18.38	2956	4.28E4	12	29
K.FRPDQPNLIFQ (+ 0.98)GGGYTTK.E	30.45	2039.01	18	5.0	680.68	18.70	3011	1.06E4	12	29	Deamidation (NQ)
D.EITSFSIPK.F	21.23	1020.55	9	− 3.8	511.28	17.77	2852	1.32E3	3	11
Total 4 peptides

Peptide sequence (previous study)	Peptide location (β chain)	References
TDEITSFSIPK	122–132	Hemperly et al. (1979)
FRPDQPNLI	133–141
EITSFSIPK	124–132

− 10lgP = Conversion form of P-value in PEAKS 8.5 software where P-value of 1% is equivalent to − 10lgP of 20
ppm delta mass is the deviation of measured mass from the theoretical mass of the peptide, in ppm, m/z mass of ions (in gas phase) per charge, RT relatively accurate prediction of peptide, PTM post translational modification, NQ = variable modification deamidation NQ (+ 0.98 Da) of asparagines (N) and glutamines (Q)