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Table 1 Hemagglutination activity of partially and purified lectins (90% fractions) extracted from fava bean, lentil, and pea on human blood groups (A, B, AB, and O)

From: Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds

Seeds Purity of lectins Conc. protein (mg/g) Total protein (mg) Total hemagglutination activity (titer) Specific hemagglutination activity (titer/mg) Fold of purification
A B AB O A B AB O A B AB O
Fava bean Partially purified 4.13 103.25 16 32 128 32 0.15 0.30 1.24 0.30 1 1 1 1
Purified 0.50 12.5 8 16 64 32 0.67 1.28 5.12 2.56 4.46 4.27 4.12 8.5
Lentil Partially purified 4.1 102.5 32 32 64 64 0.31 0.31 0.62 0.62 1 1 1 1
Purified 0.48 12 8 16 16 16 0.67 1.33 1.33 1.33 2.16 4.29 2.15 2.15
Pea Partially purified 10.64 266 8 64 64 128 0.03 0.24 0.24 0.48 1 1 1 1
Purified 1.48 37 8 32 32 64 0.22 0.86 0.86 1.73 7.33 3.58 3.58 3.60
  1. Titer, the reciprocal of the heights dilution of protein solution showing visible hemagglutination