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Table 1 Hemagglutination activity of partially and purified lectins (90% fractions) extracted from fava bean, lentil, and pea on human blood groups (A, B, AB, and O)

From: Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds

Seeds

Purity of lectins

Conc. protein (mg/g)

Total protein (mg)

Total hemagglutination activity (titer)

Specific hemagglutination activity (titer/mg)

Fold of purification

A

B

AB

O

A

B

AB

O

A

B

AB

O

Fava bean

Partially purified

4.13

103.25

16

32

128

32

0.15

0.30

1.24

0.30

1

1

1

1

Purified

0.50

12.5

8

16

64

32

0.67

1.28

5.12

2.56

4.46

4.27

4.12

8.5

Lentil

Partially purified

4.1

102.5

32

32

64

64

0.31

0.31

0.62

0.62

1

1

1

1

Purified

0.48

12

8

16

16

16

0.67

1.33

1.33

1.33

2.16

4.29

2.15

2.15

Pea

Partially purified

10.64

266

8

64

64

128

0.03

0.24

0.24

0.48

1

1

1

1

Purified

1.48

37

8

32

32

64

0.22

0.86

0.86

1.73

7.33

3.58

3.58

3.60

  1. Titer, the reciprocal of the heights dilution of protein solution showing visible hemagglutination