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Table 2 Effect of different types of medium on growth and conversion of pure IX at 25 mg IX L−1 by Eubacterium limosum

From: Exploration of isoxanthohumol bioconversion from spent hops into 8-prenylnaringenin using resting cells of Eubacterium limosum

Condition (10 H2/10 CO2/80 N2 (v/v/v))

Growth (OD600)

Molar relative IX conversion (HPLC–DAD)

BHI (reference)

++

++

MM

+

–

MM ± additions

+

–

MM, omission of glucose

–

–

MM 2 g L−1 glucose

–

–

MM 10 g L−1 glucose

+

–

BHI + zeolite (5% w/v)

++

++

MM + zeolite (5% w/v)

++

+

RCM

++

++

  1. Scoring was made relative to the incubation in BHI as positive control (‘++’: 75–100% of positive control (reference); ‘+’: 50–75% of positive control; ‘-’: < 50% or absence). 100% growth in the case of the reference corresponded with a 20-fold increase in biomass (expressed as cell dry weight) compared to the inoculum
  2. BHI brain heart infusion medium, MM minimal medium, RCM reinforced clostridial medium