Fig. 4From: Analysis and effect of conventional flasks in shaking culture of Escherichia coliReal-time CO2 and O2 concentrations in cylindrical flasks during shaking culture of E. coli K12 IFO3301. Shake-flask culture conditions were 100 and 50 mL of LB medium at 30 °C, 200 rpm with 70 mm shaking diameter, and 500-mL cylindrical flask without baffle and 500-mL baffled cylindrical flask, both equipped with breathable culture stoppers and CDMSSBack to article page