Table 2 plasmids used and generated in this study
Plasmid | Purpose | Source/Refs. |
|---|---|---|
pG-RU5R | Backbone for knockout plasmids, URA5 marker | (Millerioux et al. 2011) |
pG-URA5-PACT1-TTRP1 | Expression plasmid backbone for protein expression in M. guilliermondii (= empty vector control [pU-EV]) | (Defosse et al. 2014) |
pU-XDH-KO | Plasmid for genomic knockout of XDH1 (PGUG_05726) with URA5 | This study |
pU-XR | Plasmid for the expression of XYL1 (PGUG_00922) in M. guilliermondii (= [pU-XR]) | This study |
pU-XDH-XR-KO | Plasmid for genomic replacement of XDH1 (PGUG_01218) by XYL1 (PGUG_00922) | This study |