Production, characterization and bioinformatics analysis of l-asparaginase from a new Stenotrophomonas maltophilia EMCC2297 soil isolate

AMB Express

Table 1 Antigenic regions, their positions and sequences of E. coli, Erwinia chrysanthemi and Stenotrophomonas maltophilial-asparaginases

E. colil-asparaginase (348 aa)^a				Erwinia chrysanthemil-asparaginase^b (348 aa)				Stenotrophomonas maltophilial-asparaginase^c (335 aa)
Anti-genic region	Start	End	Sequence	Anti-genic region	Start	End	Sequence	Anti-genic region	Start	End	Sequence
1	323	333	KARVLLQLALT	1	6	20	KSLFVLVLFFVFTS	1	27	73	TPALAGDALLATVPAISDIAQIGVQSLSLVPSASLDFNLLLQVLAWA
2	224	243	PFDVSKLNELPKVGIVYNYA	2	121	142	ESAYFLHLTVKSDKPVVFVAAM	2	155	183	VHEARHVRKSSTALHAFTSPVVGPVLT
3	245	257	ASDLPAKALVDG	3	88	101	GDVVLKLSQRVL	3	145	153	RGVLVVMNA
4	118	135	AYFLDLTVKCDKPVVMVG	4	290	297	KGVVVIRS	4	294	312	MCGLLCPRKARLLLWLLTG
5	6	16	KTALAALVMGF	5	156	165	LLEAVRVAGD	5	208	239	QKIALLEATLAADTLLLEQLLPLGYEGLVIAG
6	165	174	NRGVLVVMND	6	311	333	LPGLVSDSLNPAHARILLMLALT	6	96	114	TAAFLDYLWIHDAPLVVTG
7	49	70	AGKVGVENLVNAVPQLKDIANV	7	22	34	ADKLPNIVILATG	7	242	266	AGHVSASWSNVLARIATKIPVLVGT
8	151	159	LYNAVVTAA	8	172	179	GVMVVLND	8	132	141	DATLVACTAD
9	286	298	GTAVVRSSRVPTG	9	107	114	VDGVVITH	9	82	88	DGVVVTQ
10	72	79	EQVVNIG	10	251	270	PEYLYDAAIQHGVKGIVYAG	10	186	194	GRACFLRPA
11	190	204	VATFKSVNYGPLGYI	11	226	248	TRSVFDVRGLTSLPKVDILYGYQ	11	196	205	ARMLVPLPTR
12	259	265	DGIVSAG	12	205	214	YLGVIIGNRI	12	317	331	DDLVDHLREYTLPP
13	88	94	WLTLAKK	13	52	73	AGALGVDTLINAVPEVKKLANV	13	273	279	TAQCTYG
14	18	32	GAALALPNITILAG	14	337	345	DPKVIQEYF	14	5	13	RISIATLGG
15	272	282	YKTVFDTLATA	15	274	281	GSVSVRGI
16	311	317	YGFVASG	16	303	309	GIVPPDE
17	339	345	QQIQQIF
18	104	109	GFVITH

^aE. coli FDA approved l-asparaginase (marketed under the brand name Elspar)
^bErwinia chrysanthemi (Dickeya chrysanthemi or Pectobacterium chrysanthemi) l-asparaginase (marketed under the brand name Erwinaze)
^cStenotrophomonas maltophilia (Pseudomonas maltophilia or Xanthomonas maltophilia)