Fig. 6From: Directed evolution of a transcription factor PbrR to improve lead selectivity and reduce zinc interference through dual selectionCharacterization of the growth of the mutants in response to lead and zinc under the ON condition with ampicillin for a 5 h and b 10 h, under the OFF condition with sucrose for c 5 h and d 10 h. White bar: Wild type; Grey bar: Mutant M1; Black bar: Mutant M2. Culture conditions are indicated as follows: Pb—lead; A100—ampicillin; Zn—zinc; S10—sucrose. Both lead and zinc were used at 50 µM. Ampicillin was used at 100 µg mL−1 and sucrose was used at 10 g L−1. Experiments were performed in triplicate, and data represent the averages and standard deviations of the meansBack to article page