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Fig. 6 | AMB Express

Fig. 6

From: Directed evolution of a transcription factor PbrR to improve lead selectivity and reduce zinc interference through dual selection

Fig. 6

Characterization of the growth of the mutants in response to lead and zinc under the ON condition with ampicillin for a 5 h and b 10 h, under the OFF condition with sucrose for c 5 h and d 10 h. White bar: Wild type; Grey bar: Mutant M1; Black bar: Mutant M2. Culture conditions are indicated as follows: Pb—lead; A100—ampicillin; Zn—zinc; S10—sucrose. Both lead and zinc were used at 50 µM. Ampicillin was used at 100 µg mL−1 and sucrose was used at 10 g L−1. Experiments were performed in triplicate, and data represent the averages and standard deviations of the means

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