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Table 2 Box–Behnken experimental design matrix with experimental values of xylonic acid production by Corynebacterium glutamicum ATCC 31831

From: Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess

Run order

Urea (g/L)

Xylose (g/L)

(NH4)2SO4 (g/L)

Inoculum (% v/v)

Xylonic acid (g/L)

1

11.5

60

7.5

11.25

56.119

2

11.5

90

2.5

11.25

59.792

3

11.5

30

12.5

7.5

25.061

4

4.5

30

7.5

15

21.359

5

18.5

60

2.5

15

52.481

6

11.5

30

2.5

7.5

25.061

7

11.5

90

12.5

15

58.418

8

4.5

60

12.5

11.25

30.341

9

18.5

90

7.5

15

58.795

10

4.5

90

7.5

11.25

45.749

11

18.5

60

12.5

15

48.982

12

11.5

60

7.5

15

56.018

13

11.5

60

7.5

15

56.318

14

18.5

30

7.5

11.25

28.349

15

4.5

60

2.5

7.5

28.816

  1. Maximum conversion of xylose to xylonic acid indicated in italic