Skip to main content

Table 1 Strains and plasmids used in this study

From: Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability

Strain or plasmid

Description

Remarks/references

Strains

  

 SJY4

MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0

Winston et al. (1995)

 SJY576

Leu+ Transformant of SJY4, Chr2-6 region replaced with CgLEU2

This study

 SJY577

His+ Transformants of SJY576 for left edge splitting of CgLEU2 module with CgHIS3

This study

 SH30072

Ura+ Transformant of SJY4, harboring Chr1-2 region in the mini-chromosome

Kaboli et al. (2014)

 SH30075

Ura+ Transformant of SJY4, harboring Chr2-2 region in the mini-chromosome

Kaboli et al. (2014)

 SH30077

Ura+ Transformant of SJY4, harboring Chr2-4 region in the mini-chromosome

Kaboli et al. (2014)

 SH30079

Ura+ Transformant of SJY4, harboring Chr2-6 region in the mini-chromosome

Kaboli et al. (2014)

 SH30080

Ura+ Transformant of SJY4, harboring Chr2-7 region in the mini-chromosome

Kaboli et al. (2014)

 SH30084

Ura+ Transformant of SJY4, harboring Chr3-2 region in the mini-chromosome

Kaboli et al. (2014)

Plasmids

  

 pSJ23

A derivative of pUG6 carrying URA3

Easmin et al. (2019b)

 pSJ69

loxP site-deleted p3008

Easmin et al. (2019a)

 pSJ70

loxP site deleted p3009

Easmin et al. (2019b)

 p3121

The CEN4 module containing plasmid constructed by modifying pUG6

Sugiyama et al. (2005)