Fig. 9
Spot assay of mini-chromosome harboring strains. a Represents position of spots in YPDA, -Ura and 5-FOA plate. Left side of a indicates the structure of chromosome and spots of transformants harboring Chr1-2, Chr2-2, Chr2-4, Chr2-6, Chr2-7 and Chr3-2 regions in mini-chromosome constructed by previous study (Kaboli et al. 2014). Right side of a indicates the structure of chromosome and spots of Chr2-6 (dup + rep) and Chr11-2 (dup + rep) transformants. Spots of Ura− and Ura+ strains were negative and positive control, respectively. b Represents the spotting assay of all strains in YPDA, -Ura and 5-FOA plate from day 1 to day 3. Three types of colonies were found according to the growth of transformants and these transformants were categorized as Class I, Class II and Class III. Transformants harboring Chr1-2, Chr2-2 and Chr2-7 regions belong to Class I and regions deleted in Class I transformants were considered to be essential while transformants harboring Chr3-2 region belong to Class II and the region deleted in Class II transformants was treated as non-essential. The third type of transformants harboring Chr2-4, Chr2-6 and Chr11-2 regions belong to Class III and regions deleted in Class III transformants were considered to be intrinsically essential. From each class of transformants, one representative colony was circled. Colony representing Class I transformants (harboring Chr2-2 region) was circled by red color, Class II transformants (harboring Chr3-2 region) was circled by yellow color and Class III transformants (harboring Chr2-6 region) was circled by blue color