Fig. 6

Duplication of Chr2-6 and Chr11-2 regions and checking transformants by colony PCR. Upper part of a, b represents 2 duplication modules (One module synthesized from the plasmid pSJ23 contained URA3 and another module synthesized from the plasmid p3121 contained CEN4 as a centromere) were introduced into SJY4 to duplicate Chr2-6 and Chr11-2 regions, separately. Bottom part of a, b represents gel electrophoresis of colony PCR and each lane represents independent transformants without the most left lane, which contains size marker. In a, SJP 550 and SJP 690 were used to check URA3 harboring DNA module whereas SJP 694 and SJP 697 were used to check CEN4 harboring DNA module for the duplication of Chr2-6 region, respectively. In b, SJP 692 and SJP 550 were used to check URA3 harboring DNA module whereas SJP 697 and SJP 696 were used to check CEN4 harboring DNA module for the duplication of Chr11-2 region, respectively