Fig. 3

Colony PCR analysis of replaced Chr2-2, Chr9-2 and Chr11-2 regions. Each lane represents checking of left or right edge replacement of entire Chr2-2, Chr9-2 and Chr11-2 regions in independent transformants (T1, T2, T3, T4, T5 and T6), respectively. 1 kb band was the expected band for replacement of either left or right edge of entire regions. a SJP 217 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 383 were used for checking right edge replacement of Chr2-2 region, respectively. b SJP 215 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 369 were used for checking right edge replacement of Chr9-2 region, respectively. c SJP 219 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 387 were used for checking right edge replacement of Chr11-2 region, respectively