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Fig. 2 | AMB Express

Fig. 2

From: Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability

Fig. 2

Colony PCR analysis of replaced sub-regions of Chr2-6 region. Each lane represents checking of left or right edge replacement of Chr2-6 sub-regions in independent transformants (T1, T2, T3, T4, T5 and T6). M represents size marker (Gene Ladder Wide 2, Nippon Gene, Toyama, Japan). A common set of primers (SJP 121 and SJP 242) was used in all PCR verification experiments to amplify the 0.67 kb CNE1 gene on Chromosome 1 as an internal control. 1 kb band was the expected band for replacement of either left or right edge of sub-regions. a SJP 118 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 384 were used for checking right edge replacement of A1 + A2 sub-regions, respectively. b SJP 390 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 385 were used for checking right edge replacement of B1 + B2 sub-regions, respectively. c SJP 118 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 457 were used for checking right edge replacement of A1 + A2 + B1 sub-regions, respectively. d SJP 427 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 385 were used for checking right edge replacement of A2 + B1 + B2 sub-regions, respectively. e SJP 118 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 479 were used for checking right edge replacement of A1 + A2 + B1 (Ex) sub-regions, respectively. f SJP 483 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 385 were used for checking right edge replacement of A2 (Ex) + B1 + B2 sub-regions, respectively. g SJP 118 and SJP 119 were used for checking left edge whereas SJP 127 and SJP 385 were used for checking right edge replacement of entire Chr2-6 region, respectively

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