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Fig. 3 | AMB Express

Fig. 3

From: A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease

Fig. 3

a SDS-PAGE analysis of the expression of surface-displayed SUMO-mCherry and Ulp1 induced by IPTG and the induced cells after incubation in the presence of 200 μg/mL trypsin at 37 °C for 1 h. b Protein expression in different fractions of induced cells. Equal volumes of fractionated samples were used for SDS-PAGE analyses. C: cytoplasmic fraction; I: inner membrane fraction; O: outer membrane fraction. The results are representative of three independent experiments. c Immunolabeling analysis of induced intact cells using anti-His antibody. After three washes to remove unbound secondary antibody, the cells were incubated in ECL substrate solution for 10 min to develop the chemiluminescence. The luminescence intensity was measured at 425 nm using a microplate reader. Data are means of three independent experiments, error bars represent standard deviation

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