Transgenic plant generated by RNAi-mediated knocking down of soybean Vma12 and soybean mosaic virus resistance evaluation

AMB Express

Table 1 Results from screening positive plants in T₀ and T₁GmVma12 transgenic generations

Serial number	Explant number	Rooting plants	Seedling number	Number of positive T₀ plants^a	Number of T₁ plants	Number of positive T₁ plants^a
1	103	13	8	8	37	32
2	135	27	9	8	64	58
3	256	9	4	3	24	21
4	210	8	6	4	49	27
5	185	9	5	5	18	16
6	238	10	5	5	17	6
Total	1127	76	37	33	209	160

^aAll of the positive plants were confirmed by leaf-painting assay, PCR verification and LibertyLink^® strip detection