Table 4 Experimental conditions and results of experimental setup I for protease production
Parameters | A1 | A2 | A3 | A4 | A5 | A6 | A7 | A8 | A9 | A10 |
|---|---|---|---|---|---|---|---|---|---|---|
Temperature/°C | 28 | 26 | 26 | 30 | 30 | 28 | 30 | 26 | 30 | 26 |
pH | 6 | 4 | 8 | 4 | 8 | 6 | 8 | 4 | 4 | 8 |
WB +SM ratio (in g) | 4:1 | 3.5:1.5 | 4.5:0.5 | 4.5:0.5 | 3.5:1.5 | 4:1 | 4.5:0.5 | 4.5:0.5 | 3.5:1.5 | 3.5:1.5 |
Protease activity (U/gds) (Cycle 1) | 406.17 | 158.97 | 48.63 | 88.80 | 153.34 | 412.79 | 189.32 | 107.69 | 110.26 | 99.30 |
Protease Activity (U/gds) (Cycle 2) | 389.89 | 129.93 | 50.12 | 98.69 | 134.86 | 384.16 | 200.94 | 122.02 | 103.69 | 110.53 |
Differences | 16.28 | 29.04 | − 1.49 | − 9.89 | 18.48 | 28.63 | − 11.62 | − 14.33 | 6.57 | − 11.23 |
Average | 398.03 | 144.45 | 49.38 | 93.74 | 144.10 | 398.47 | 195.13 | 114.85 | 106.97 | 104.91 |