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Table 4 Experimental conditions and results of experimental setup I for protease production

From: Combined ANN/EVOP Factorial Design Approach for Media Screening for Cost-effective Production of Alkaline Proteases from Rhizopus oryzae (SN5)/NCIM-1447 under SSF

ParametersA1A2A3A4A5A6A7A8A9A10
Temperature/°C28262630302830263026
pH6484868448
WB +SM ratio (in g)4:13.5:1.54.5:0.54.5:0.53.5:1.54:14.5:0.54.5:0.53.5:1.53.5:1.5
Protease activity (U/gds) (Cycle 1)406.17158.9748.6388.80153.34412.79189.32107.69110.2699.30
Protease Activity (U/gds) (Cycle 2)389.89129.9350.1298.69134.86384.16200.94122.02103.69110.53
Differences16.2829.04− 1.49− 9.8918.4828.63− 11.62− 14.336.57− 11.23
Average398.03144.4549.3893.74144.10398.47195.13114.85106.97104.91