Table 2 Primers used in this study
Primers | Purpose | Sequence (5′–3′) |
|---|---|---|
orf35-attB-F/R | Amplification of orf35-attB for construction of pSET1139-attB | CTGCAGGTCGACTCTAGACCTCGATCCGGACCACGAGCAC CCCGGGGAGCCCAAGGGCACGCCCTGGCACCCGCACCGCGGTCAATATCCGTACCGGCT |
orf36-attB-F/R | Amplification of orf36-attB for construction of pSET1139-attB | TGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACTCACCGGTCGATCAAGGG GCGCGCGGCCGCGGATCCCGAGACGCTGACCACTGGGCAG |
kasO*P-F/R | Amplification of kasO*P promoter for construction of pSET152-vcm8 | CAAGCTTGGGCTGCAGGTCGACTCTAGATGTTCACATTCGAACCGTCTC ACCACATCGAAGTCTTCGACCGACATATGGACACTCCTTACTTAGACTG |
vcm8-F/R | Amplification of vcm8 for construction of pSET152-vcm8 | GTCTAAGTAAGGAGTGTCCATATGTCGGTCGAAGATTTCGATGTTGTG TCGATATCGCGCGCGGCCGCGGATCCTCAGGCCGGGTGGTGCGGCAGC |
orf10-F/R | Amplification of orf10 for construction of pSET152-orf10 | CAGTCTAAGTAAGGAGTGTCCATATGTCGGTCGAAGACTTCGATGT TCGATATCGCGCGCGGCCGCGGATCCTCATGCCGGATGGTGCGGCAGCC |
orf11-F/R | Amplification of orf11 for construction of pSET152-orf11 | GTCTAAGTAAGGAGTGTCCATATGCGCGTGTTGATTACGGGGTGTG TCGATATCGCGCGCGGCCGCGGATCCTCAGGCGGGAACAGTCGGC |
orf12-F/R | Amplification of orf12 for construction of pSET152-orf12 | GTCTAAGTAAGGAGTGTCCATATGCGTGTGCTGTTGGCGACGTGTG TCGATATCGCGCGCGGCCGCGGATCCTTACGCGGAAACAGTCGGC |
orf13-F/R | Amplification of orf13 for construction of pSET152-orf13 | GTCTAAGTAAGGAGTGTCCATATGCGTGTGTTGTTGTCGACGGCTG TCGATATCGCGCGCGGCCGCGGATCCTTACGCGAGAACAGCCGAC |
orf10–orf11-F/R | Amplification of orf10–orf11 for construction of pSET152-orf10–orf11 | GTCTAAGTAAGGAGTGTCCATATGTCGGTCGAAGACTTCGATGT TCGATATCGCGCGCGGCCGCGGATCCATGTCAGGCGGGAACAGTCGGCTTTT |
attB-F/R | Amplification of artificial attB site in K. aridum | CCGCGGTGCGGGTGCCAGGGCGTGC CTCTCCCCTCTGATCGAGGACCAG |