Table 1 Bacterial strains and plasmids
Strains or plasmids | Description | Source |
|---|---|---|
Plasmid vectors | ||
pSET152 | acc(3)IV, oriTRK2, φC31, int | Bierman et al. (1992) |
pKC1139 | acc(3)IV, pSG5, oriTRK2 | Bierman et al. (1992) |
pSBJ153 | acc(3)IV, oriTRK2, kasO*P promoter | Wang et al. (2013) |
pKC1139-attB | acc(3)IV, pSG5, oriTRK2, attB | This study |
pSET152-vcm8 | acc(3)IV, oriTRK2, Aprar, kasO*P, vcm8 | This study |
pSET152-orf10 | acc(3)IV, oriTRK2, Aprar, kasO*P, orf10 | This study |
pSET152-orf11 | acc(3)IV, oriTRK2, Aprar, kasO*P, orf11 | This study |
pSET152-orf12 | acc(3)IV, oriTRK2, Aprar, kasO*P, orf12 | This study |
pSET152-orf13 | acc(3)IV, oriTRK2, Aprar, kasO*P, orf13 | This study |
pSET152-orf10–orf11 | acc(3)IV, oriTRK2, Aprar, kasO*P, orf10–orf11 | This study |
Strains | ||
E. coli DH5α | Host for general cloning | FENGHBIO, China |
E. coli ET12567/pUZ8002 | Donor strain for intergenetic conjugation between E. coli and Streptomyces, Kanr, Chlr | FENGHBIO, China |
A. orientalis ATCC 43491 | Vancomycin-producing the parental strain | ATCC |
K. aridum SIPI-3927 (CGMCC 4.7675) | A82846B-producing strain | CGMCC |
SIPI-3927-attB | SIPI-3927, attB | This study |
SIPI-pSET152 | SIPI-3927-attB, pSET152 | This study |
SIPI-3927-C1 | SIPI-3927-attB, pSET152-vcm8 | This study |
SIPI-3927-C2 | SIPI-3927-attB, pSET152-orf10 | This study |
SIPI-3927-C3 | SIPI-3927-attB, pSET152-orf11 | This study |
SIPI-3927-C4 | SIPI-3927-attB, pSET152-orf12 | This study |
SIPI-3927-C5 | SIPI-3927-attB, pSET152-orf13 | This study |
SIPI-3927-C6 | SIPI-3927-attB, pSET152-orf10–orf11 | This study |