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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Enhancing A82846B production by artificial attB-assisted overexpression of orf10–orf11 genes in Kibdelosporangium aridum SIPI-3927

Fig. 2

Homologous recombination to construct SIPI-3927-attB strain containing attB site. a Single exchange screening of recombinant strains containing apramycin resistance. b Double exchange screening of recombinant strains with loss of apramycin resistance. c, d PCR amplification results of double exchange strains. M, maker; 1–2, double-exchange strain containing attB site; 3, double-exchange strain without attB site; 4, parent strain

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