Fig. 6

The effect of promoters on display efficiencies. The differences between the GAP promoter and GAL1 promoter in a display systems 2 and 4 and b display systems 3 and 5 were compared. The data were extracted from Fig. 3. The fluorescence intensity of AF488 represents the relative amount of displayed nanobodies on a yeast cell, while that of AF647 represents the relative amount of functional nanobodies. The Q2 region represents yeast cells with strong AF488 and AF647 signals (see “Materials and methods”). a The influence of the promoters when the anchor protein was α-agglutinin. Display systems 2 and 4 were compared. The fluorescence intensity was normalized against that of display system 2 at 25 °C. b The influence of the promoters when the anchor protein was 649-stalk. Display systems 3 and 5 were compared. The fluorescence intensity was normalized against that of display system 3 at 25 °C. The bars indicate the means ± standard deviations of the three biological replicates. *P < 0.05, **P < 0.01, Tukey’s test