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Fig. 5 | AMB Express

Fig. 5

From: Evaluation of the yeast surface display system for screening of functional nanobodies

Fig. 5

The effect of anchor proteins on display efficiencies. To analyze the difference between α-agglutinin and 649-stalk, a display systems 2 and 3 and b display systems 4 and 5 were compared. The data were extracted from Fig. 3. The fluorescence intensity of AF488 represents the relative amount of displayed nanobodies on a yeast cell, while that of AF647 represents the relative amount of functional nanobodies. a The influences of the anchor proteins under the GAP promoter in display systems 2 and 3. The data were normalized against the fluorescence intensity of display system 2 at 25 °C. b The influences of the anchor proteins under the GAL1 promoter in display systems 4 and 5. The data were normalized against the fluorescence intensity of display system 4 at 25 °C. The bars indicate the means ± standard deviations of the three biological replicates. *P < 0.05, **P < 0.01, Tukey’s test

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