Fig. 4
From: Evaluation of the yeast surface display system for screening of functional nanobodies

The effect of secretion signals on display efficiencies. To analyze the difference between the glucoamylase secretion signal (G. A. s. s.) and improved α-factor secretion signal (α-factor s. s.), the fluorescence intensities of display system 1 and display system 2 were compared. The data were extracted from Fig. 3. The fluorescence intensity of AF488 represents the relative amount of displayed nanobodies on a yeast cell, while that of AF647 represents the relative amount of functional nanobodies. The bars indicate the means ± standard deviations of the three biological replicates. The data were normalized against the fluorescence intensity of display system 1 at 25 °C. *P < 0.05, **P < 0.01, Tukey’s test