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Fig. 3 | AMB Express

Fig. 3

From: Evaluation of the yeast surface display system for screening of functional nanobodies

Fig. 3

Comparison of the display efficiencies after 12-h culture. Each yeast sample was stained using anti-HA tag monoclonal antibody and AF488-conjugated anti-mouse secondary antibody and AF647-labeled lysozyme. a The ratio of yeast cells in the Q2 region, which represents yeast cells with strong AF488 and AF647 signals (see “Materials and methods”). b The mean fluorescence intensity of yeast cells in the Q2 region. The fluorescence intensity of AF488 represents the relative amount of displayed nanobodies on a yeast cell, while that of AF647 represents the relative amount of functional nanobodies. The bars indicate the means ± standard deviations of the three biological replicates

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