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Fig. 2 | AMB Express

Fig. 2

From: Evaluation of the yeast surface display system for screening of functional nanobodies

Fig. 2

The effects of culture conditions on display efficiencies. a Schematic representation of the experimental scheme. Yeast cells were cultured at 25 °C or 30 °C for 72 h. Each yeast sample was stained using anti-HA tag mouse monoclonal antibody and AF488-conjugated anti-mouse monoclonal antibody as a secondary antibody to quantify the relative amount of displayed nanobodies. Alexa Fluor® 647 (AF647)-labeled lysozyme was also used to quantify the relative amount of functional nanobodies. NB, anti-hen egg-white lysozyme nanobody; LYS, hen egg white-lysozyme. b Representative images of the flow cytometry analysis of the yeast cells with display system 1. The Q2 region (%) shows the ratio of yeast cells with strong AF488 and AF647 signals (see Materials and Methods). The display efficiencies of c display system 1, d display system 2, e display system 3, f display system 4, and g display system 5. The bar graphs display the mean fluorescence intensity of yeast cells in the Q2 region, and the black lines show the ratio of yeast cells in the Q2 region

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