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Table 2 Effect of chemical reagents on recombinant enzymes

From: Recombinant expression and characterization of two glycoside hydrolases from extreme alklinphilic bacterium Cellulomonas bogoriensis 69B4T

Chemicals

Relative activity (%)

Cel5A

Cel9A

1%

10%

1%

10%

Methanol

92.7 + 3.8

87.5 + 0.6

98.9 ± 3.5

26.2 ± 3.4

Ethanol

96.7 + 1.6

86.1 + 2.3

73.4 ± 2.4

15.9 ± 0.71.7

Glycerin

87.5 + 0.9

86. 8 + 1.3

110.7 ± 1.7

111.3 ± 2.3

Isopropanol

93.7 + 0.3

87.4 + 0.1

118.7 ± 1.8

15.7 ± 1.9

SDS

66.3 + 0.4

4.6 + 0.8

26.3 ± 1.2

8.4 ± 1.7

Tween-80

100.1 + 0.5

102.6 + 2.6

101.7 ± 1.3

115.7 ± 1.8

Triton X-100

94.1 + 2.4

94.3 + 1.0

113.3 ± 4.0

63.5 ± 0.8