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Table 1 Effect of metals on recombinant enzymes

From: Recombinant expression and characterization of two glycoside hydrolases from extreme alklinphilic bacterium Cellulomonas bogoriensis 69B4T

Metal ions

Relative activity (%)

Cel5A

Cel9A

1 mM

10 mM

1 mM

10 mM

Fe2+

132.5 ± 1.6

39.8 ± 6.0

129.0 ± 0.5

40.2 ± 7.1

Fe3+

75.8 ± 2.7

0

83.3 ± 1.7

21.0 ± 0.6

Cu2+

77.6 ± 0.4

45.4 ± 1.5

116.5 ± 0.5

35.3 ± 4.7

Ca2+

102.9 ± 2.2

113.5 ± 1.3

116.1 ± 1.5

71.7 ± 2.2

Na+

101.9 ± 1.1

101.8 ± 2.1

117.8 ± 1.5

102.3 ± 1.6

K+

103.7 ± 3.8

104.8 ± 0.3

105.6 ± 0.6

103.7 ± 1.7

Ni2+

107.64 ± 2.0

110.9 ± 1.6

81.5 ± 2.2

28.5 ± 4.1

Mn2+

71.3 ± 4.5

0

43.9 ± 4.3

56.8 ± 1.0

Co2+

75.5 ± 0.1

38.1 ± 1.5

76.0 ± 3.5

38.9 ± 6.3

Mg2+

103.3 ± 3.8

145.1 ± 0.4

95.3 ± 3.4

104.5 ± 1.8

Zn2+

93.4 ± 3.7

97.4 ± 2.6

72.6 ± 0.5

47.1 ± 2.0

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