Fig. 2From: Recombinant expression and characterization of two glycoside hydrolases from extreme alklinphilic bacterium Cellulomonas bogoriensis 69B4TSDS-PAGE and zymogram of recombinant enzyme Cel5A (a) and Cel9A (b). Lane M molecular mass markers; a Lane 1 total protein of cells without induction, Lane 2 total protein of cells induced with IPTG, Lane 3 purified recombinant Cel5A, Lane 4 zymogram of purified Cel5A; b Lane 1 total protein of cells without induction, Lane 2 total protein of cells induced with IPTG, Lane 3 purified MBP fusion protein, Lane 4 fusion protein digestion by Factor Xa, Lane 5 purified recombinant Cel9A, Lane 6 zymogram of purified Cel9ABack to article page