Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

AMB Express

Table 4 Analysis of variance (ANOVA) for the response surface of full quadratic model used for optimization of four categories of additives (A, cane molasses; B, combined nitrogenous mixture; C, casein hydrolysate; D, glucose)

Source	Sum of squares	Degree of freedom	Mean square	F Value	p-value Prob > F
Model	6.37	14	0.46	343.73	< 0.0001
A-cane molasses	0.76	1	0.76	571.23	< 0.0001
B-combined nitrogenous mixture	1.07	1	1.07	805.92	< 0.0001
C-casein hydrolysate	1.49	1	1.49	1125.63	< 0.0001
D-glucose	0.14	1	0.14	106.57	< 0.0001
AB	0.34	1	0.34	258.53	< 0.0001
AC	6.400E−003	1	6.400E−003	4.83	0.0466
AD	8.100E−003	1	8.100E−003	6.12	0.0279
BC	0.60	1	0.60	453.74	< 0.0001
BD	0.14	1	0.14	106.23	< 0.0001
CD	0.073	1	0.073	55.07	< 0.0001
A²	0.26	1	0.26	198.31	< 0.0001
B²	1.60	1	1.60	1209.98	< 0.0001
C²	0.43	1	0.43	328.40	< 0.0001
D²	0.17	1	0.17	125.91	< 0.0001
Residual	0.017	13	1.324E−003
Lack of fit	0.016	10	1.641E−003	6.15	0.0808