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Table 1 Box–Behnken design of experiment using four variables (A, cane molasses; B, CSL; C, WGE; D, and cheese whey)

From: Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

Std

Run

Factor (A): cane molasses [% (v/v)]

Factor (B): CSL [% (v/v)]

Factor (C): WGE [% (v/v)]

Factor (D): cheese whey [% (w/v)]

Dry biomass (g/L)

3

1

10

7.5

35

3

9.34

13

2

12.5

3

10

3

9.19

9

3

10

5.25

35

1

9.5

15

4

12.5

3

70

3

9.17

24

5

12.5

7.5

35

5

7.91

8

6

12.5

5.25

70

5

8.21

12

7

15

5.25

35

5

7.59

28

8

12.5

5.25

35

3

9.12

2

9

15

3

35

3

8.45

17

10

10

5.25

10

3

9.7

25

11

12.5

5.25

35

3

9.12

11

12

10

5.25

35

5

9.1

6

13

12.5

5.25

70

1

8.94

23

14

12.5

3

35

5

8.51

18

15

15

5.25

10

3

8.41

26

16

12.5

5.25

35

3

9.15

5

17

12.5

5.25

10

1

9.08

14

18

12.5

7.5

10

3

8.82

20

19

15

5.25

70

3

8.26

7

20

12.5

5.25

10

5

8.35

10

21

15

5.25

35

1

8.57

19

22

10

5.25

70

3

9.59

22

23

12.5

7.5

35

1

8.88

27

24

12.5

5.25

35

3

9.11

16

25

12.5

7.5

70

3

8.56

4

26

15

7.5

35

3

8.07

1

27

10

3

35

3

9.81

21

28

12.5

3

35

1

9.01

  1. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00–0.09