AMB Express

Table 1 Effect of metal ions and chemical on the cellulase activity of Cel-A5

From: Cloning and characterization of low-temperature adapted GH5-CBM3 endo-cellulase from Bacillus subtilis 1AJ3 and their application in the saccharification of switchgrass and coffee grounds

Metal ion and chemicals	Relative activity (%)
Metal ion and chemicals	10 mM	5 mM	1 mM
Na⁺	98.37 ± 0.72	98.67 ± 6.77	88.87 ± 0.92
K⁺	101.14 ± 0.69	94.08 ± 2.44	107.63 ± 3.93
Cu²⁺	15.77 ± 1.71	79.05 ± 0.54	115.23 ± 0.58
Mg²⁺	89.38 ± 3.07	88.74 ± 2.29	97.11 ± 0.63
Zn²⁺	85.71 ± 1.63	95.80 ± 3.35	119.36 ± 0.43
Ca²⁺	97.03 ± 1.02	88.44 ± 0.39	111.87 ± 0.57
Mn²⁺	74.25 ± 0.77	68.72 ± 2.40	73.91 ± 3.97
Fe²⁺	54.24 ± 0.37	106.77 ± 2.09	123.59 ± 0.67
Fe³⁺	3.91 ± 1.25	39.40 ± 6.74	99.16 ± 3.91
Al³⁺	1.78 ± 0.79	60.28 ± 0.67	104.19 ± 2.42
Co²⁺	110.15 ± 1.34	124.02 ± 5.36	140.38 ± 3.25
Ti⁴⁺	7.08 ± 0.24	18.85 ± 1.43	93.57 ± 1.07
Cs⁺	84.34 ± 0.83	92.50 ± 2.52	76.34 ± 2.56
Li⁺	91.89 ± 4.98	106.11 ± 2.12	76.84 ± 2.64
Ni²⁺	86.66 ± 3.12	105.32 ± 6.08	95.68 ± 1.47
Rb²⁺	85.18 ± 4.67	99.82 ± 1.52	99.61 ± 3.06
SDS	45.91 ± 0.90	29.04 ± 0.25	84.89 ± 1.65

Activity without any metal ion set as 100%

Back to article page