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Table 1 Effect of metal ions and chemical on the cellulase activity of Cel-A5

From: Cloning and characterization of low-temperature adapted GH5-CBM3 endo-cellulase from Bacillus subtilis 1AJ3 and their application in the saccharification of switchgrass and coffee grounds

Metal ion and chemicalsRelative activity (%)
10 mM5 mM1 mM
Na+98.37 ± 0.7298.67 ± 6.7788.87 ± 0.92
K+101.14 ± 0.6994.08 ± 2.44107.63 ± 3.93
Cu2+15.77 ± 1.7179.05 ± 0.54115.23 ± 0.58
Mg2+89.38 ± 3.0788.74 ± 2.2997.11 ± 0.63
Zn2+85.71 ± 1.6395.80 ± 3.35119.36 ± 0.43
Ca2+97.03 ± 1.0288.44 ± 0.39111.87 ± 0.57
Mn2+74.25 ± 0.7768.72 ± 2.4073.91 ± 3.97
Fe2+54.24 ± 0.37106.77 ± 2.09123.59 ± 0.67
Fe3+3.91 ± 1.2539.40 ± 6.7499.16 ± 3.91
Al3+1.78 ± 0.7960.28 ± 0.67104.19 ± 2.42
Co2+110.15 ± 1.34124.02 ± 5.36140.38 ± 3.25
Ti4+7.08 ± 0.2418.85 ± 1.4393.57 ± 1.07
Cs+84.34 ± 0.8392.50 ± 2.5276.34 ± 2.56
Li+91.89 ± 4.98106.11 ± 2.1276.84 ± 2.64
Ni2+86.66 ± 3.12105.32 ± 6.0895.68 ± 1.47
Rb2+85.18 ± 4.6799.82 ± 1.5299.61 ± 3.06
SDS45.91 ± 0.9029.04 ± 0.2584.89 ± 1.65
  1. Activity without any metal ion set as 100%