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Table 4 Identified proteins and peptides from Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

BandGenBank accession codeaProtein descriptionBLAST analysisDeepLoc predictionMw based on AA sequence [kDa]Mw based on SDS-PAGE [kDa]# of peptidesbSequence coverage [%]bPositionbPeptide sequencebpb
1PKK45954.1
PTB58544.1/XP_02477822.1
Hypothetical proteinSubtilisin-like serine proteaseExtracellular± 93± 7011[668–679]LALQTVTLPAGK6.6·10−4
2PKK54770.1Hypothetical proteinAmidaseExtracellular± 61± 6047[138–146]DAGAVLFGK1.0·10−3
 PTB50895.1/XP_024770572.1       [147–15]AALSEWADMR9.8·10−4
 PNP55812.1       [315–324]ILTSILTLIK5.7·10−4
 KKO98739.1       [518–527]YASAIEDLQK0.025
3KKP07817.1
PKK51292.1
PNP54665.1
PTB57154.1/XP_024776831.1
Hypothetical proteinGlycoside hydrolase family 92 or α-1,2-mannosidaseExtracellular± 85± 5511[247–257]EIPNFNFESVR0.025
4#PKK55157.1Hypothetical proteinSarcosine oxidasePeroxisome± 49± 47415[31–51]VVVLEQNNFFNHAGSSNDLAR4.2·10−6
 PTB52097.1/XP_024771774.1       [64–74]EAMALWDDLER6.2·10−3
         [138–156]YIGLFAPDNGVINVQLLLR1.0·10−5
         [300–317]LTSTINPEDIADTQEFIR0.022
 PKK45954.1
PTB58544.1/XP_02477822.1
Hypothetical proteinSubtilisin-like serine proteaseExtracellular± 93± 4711[668–679]LALQTVTLPAGK1.7·10−3
5#PKK55157.1Hypothetical proteinSarcosine oxidasePeroxisome± 49± 41310[31–51]VVVLEQNNFFNHAGSSNDLAR9.4·10−3
 PTB52097.1/XP_024771774.1       [138–156]YIGLFAPDNGVINVQLLLR4.8·10−4
         [284–291]IAVDAATR0.028
 ACM42428.1β-1,6-glucanaseNAExtracellular± 48± 4112[41–51]FEPALASGITK7.5·10−3
 KKP05604.1          
 PKK48835.1/XP_024773174.1          
  1. These peptides were analyzed via BLAST and DeepLoc prediction to verify whether the protein found via BLAST corresponds to the expected location of the proteins (extracellular). Most proteins were identified to be extracellular, except for sarcosine oxidase (peroxisome)
  2. #Number of 13C = 1, default: number of 13C = 0; NA not applicable; Italics indicate protein hits that are significant but with low reliability
  3. aIdentity between protein hits ≥ 97%
  4. bReported for the first GenBank accession code