Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Heshof, Ruud; Visscher, Bram; van de Zilver, Eric; van de Vondervoort, Rick; van Keulen, Femke; Delahaije, Roy J. B. M.; Wind, Richèle D.

doi:10.1186/s13568-020-00974-z

AMB Express

Table 4 Identified proteins and peptides from Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Band	GenBank accession code^a	Protein description	BLAST analysis	DeepLoc prediction	M_w based on AA sequence [kDa]	M_w based on SDS-PAGE [kDa]	# of peptides^b	Sequence coverage [%]^b	Position^b	Peptide sequence^b	p^b
1	PKK45954.1 PTB58544.1/XP_02477822.1	Hypothetical protein	Subtilisin-like serine protease	Extracellular	± 93	± 70	1	1	[668–679]	LALQTVTLPAGK	6.6·10⁻⁴
2	PKK54770.1	Hypothetical protein	Amidase	Extracellular	± 61	± 60	4	7	[138–146]	DAGAVLFGK	1.0·10⁻³
	PTB50895.1/XP_024770572.1								[147–15]	AALSEWADMR	9.8·10⁻⁴
	PNP55812.1								[315–324]	ILTSILTLIK	5.7·10⁻⁴
	KKO98739.1								[518–527]	YASAIEDLQK	0.025
3	KKP07817.1 PKK51292.1 PNP54665.1 PTB57154.1/XP_024776831.1	Hypothetical protein	Glycoside hydrolase family 92 or α-1,2-mannosidase	Extracellular	± 85	± 55	1	1	[247–257]	EIPNFNFESVR	0.025
4^#	PKK55157.1	Hypothetical protein	Sarcosine oxidase	Peroxisome	± 49	± 47	4	15	[31–51]	VVVLEQNNFFNHAGSSNDLAR	4.2·10⁻⁶
	PTB52097.1/XP_024771774.1								[64–74]	EAMALWDDLER	6.2·10⁻³
									[138–156]	YIGLFAPDNGVINVQLLLR	1.0·10⁻⁵
									[300–317]	LTSTINPEDIADTQEFIR	0.022
	PKK45954.1 PTB58544.1/XP_02477822.1	Hypothetical protein	Subtilisin-like serine protease	Extracellular	± 93	± 47	1	1	[668–679]	LALQTVTLPAGK	1.7·10⁻³
5^#	PKK55157.1	Hypothetical protein	Sarcosine oxidase	Peroxisome	± 49	± 41	3	10	[31–51]	VVVLEQNNFFNHAGSSNDLAR	9.4·10⁻³
	PTB52097.1/XP_024771774.1								[138–156]	YIGLFAPDNGVINVQLLLR	4.8·10⁻⁴
									[284–291]	IAVDAATR	0.028
	ACM42428.1	β-1,6-glucanase	NA	Extracellular	± 48	± 41	1	2	[41–51]	FEPALASGITK	7.5·10⁻³
	KKP05604.1
	PKK48835.1/XP_024773174.1

These peptides were analyzed via BLAST and DeepLoc prediction to verify whether the protein found via BLAST corresponds to the expected location of the proteins (extracellular). Most proteins were identified to be extracellular, except for sarcosine oxidase (peroxisome)
^#Number of ¹³C = 1, default: number of ¹³C = 0; NA not applicable; Italics indicate protein hits that are significant but with low reliability
^aIdentity between protein hits ≥ 97%
^bReported for the first GenBank accession code

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