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Table 4 Identified proteins and peptides from Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Band GenBank accession codea Protein description BLAST analysis DeepLoc prediction Mw based on AA sequence [kDa] Mw based on SDS-PAGE [kDa] # of peptidesb Sequence coverage [%]b Positionb Peptide sequenceb pb
1 PKK45954.1
PTB58544.1/XP_02477822.1
Hypothetical protein Subtilisin-like serine protease Extracellular ± 93 ± 70 1 1 [668–679] LALQTVTLPAGK 6.6·10−4
2 PKK54770.1 Hypothetical protein Amidase Extracellular ± 61 ± 60 4 7 [138–146] DAGAVLFGK 1.0·10−3
  PTB50895.1/XP_024770572.1         [147–15] AALSEWADMR 9.8·10−4
  PNP55812.1         [315–324] ILTSILTLIK 5.7·10−4
  KKO98739.1         [518–527] YASAIEDLQK 0.025
3 KKP07817.1
PKK51292.1
PNP54665.1
PTB57154.1/XP_024776831.1
Hypothetical protein Glycoside hydrolase family 92 or α-1,2-mannosidase Extracellular ± 85 ± 55 1 1 [247–257] EIPNFNFESVR 0.025
4# PKK55157.1 Hypothetical protein Sarcosine oxidase Peroxisome ± 49 ± 47 4 15 [31–51] VVVLEQNNFFNHAGSSNDLAR 4.2·10−6
  PTB52097.1/XP_024771774.1         [64–74] EAMALWDDLER 6.2·10−3
          [138–156] YIGLFAPDNGVINVQLLLR 1.0·10−5
          [300–317] LTSTINPEDIADTQEFIR 0.022
  PKK45954.1
PTB58544.1/XP_02477822.1
Hypothetical protein Subtilisin-like serine protease Extracellular ± 93 ± 47 1 1 [668–679] LALQTVTLPAGK 1.7·10−3
5# PKK55157.1 Hypothetical protein Sarcosine oxidase Peroxisome ± 49 ± 41 3 10 [31–51] VVVLEQNNFFNHAGSSNDLAR 9.4·10−3
  PTB52097.1/XP_024771774.1         [138–156] YIGLFAPDNGVINVQLLLR 4.8·10−4
          [284–291] IAVDAATR 0.028
  ACM42428.1 β-1,6-glucanase NA Extracellular ± 48 ± 41 1 2 [41–51] FEPALASGITK 7.5·10−3
  KKP05604.1           
  PKK48835.1/XP_024773174.1           
  1. These peptides were analyzed via BLAST and DeepLoc prediction to verify whether the protein found via BLAST corresponds to the expected location of the proteins (extracellular). Most proteins were identified to be extracellular, except for sarcosine oxidase (peroxisome)
  2. #Number of 13C = 1, default: number of 13C = 0; NA not applicable; Italics indicate protein hits that are significant but with low reliability
  3. aIdentity between protein hits ≥ 97%
  4. bReported for the first GenBank accession code