Table 3 Total oil content remaining in 30% (v/v) fat Schwanniomyces occidentalis pellet after using tailor-made enzymes in a variety of conditions in temperature, pH, and time
Sample | pH | Temperature (°C) | Time (h) | Average oil content in pellet (mg) |
|---|---|---|---|---|
1 | 3.0 | 10 | 1 | 67.59 ± 4.02 |
2 | 3.0 | 10 | 4 | 67.44 ± 2.11 |
3 | 3.0 | 10 | 24 | 19.69 ± 3.26 |
4 | 3.0 | 40 | 1 | 64.61 ± 2.33 |
5 | 3.0 | 40 | 4 | 65.82 ± 1.66 |
6 | 3.0 | 40 | 24 | 0.90 ± 0.07 |
7 | 3.0 | 70 | 1 | 58.22 ± 2.46 |
8 | 3.0 | 70 | 4 | 62.12 ± 1.97 |
9 | 3.0 | 70 | 24 | 63.27 ± 0.46 |
10 | 5.0 | 10 | 1 | 69.58 ± 0.67 |
11 | 5.0 | 10 | 4 | 71.50 ± 0.42 |
12 | 5.0 | 10 | 24 | 1.07 ± 0.27 |
13 | 5.0 | 40 | 1 | 55.02 ± 2.04 |
14 | 5.0 | 40 | 4 | 0.00 ± 0.00 |
15 | 5.0 | 40 | 24 | 0.04 ± 0.05 |
16 | 5.0 | 70 | 1 | 58.82 ± 1.13 |
17 | 5.0 | 70 | 4 | 56.89 ± 0.52 |
18 | 5.0 | 70 | 24 | 0.40 ± 0.18 |
19 | 7.0 | 10 | 1 | 67.05 ± 1.70 |
20 | 7.0 | 10 | 4 | 66.17 ± 2.34 |
21 | 7.0 | 10 | 24 | 70.48 ± 2.81 |
22 | 7.0 | 40 | 1 | 66.57 ± 2.32 |
23 | 7.0 | 40 | 4 | 70.64 ± 3.00 |
24 | 7.0 | 40 | 24 | 66.49 ± 2.50 |
25 | 7.0 | 70 | 1 | 68.59 ± 3.47 |
26 | 7.0 | 70 | 4 | 74.30 ± 3.77 |
27 | 7.0 | 70 | 24 | 67.90 ± 0.79 |
Controls—demi water, no enzymes | ||||
28 | 3.0 | 70 | 24 | 74.91 ± 0.15 |
29 | 5.0 | 70 | 24 | 75.07 ± 0.24 |
30 | 7.0 | 70 | 24 | 61.24 ± 0.05 |