AMB Express

Table 2 Overview of the different pretreatment procedures taken in this study

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Sample number	Method/added chemical	Effect
1	1 M NaCl	Osmotic pressure
2	1 M NaOH	Strong base
3	1 M NH₄OH	Weak base
4	1 M HCl	Strong acid
5	1 M H₃PO₄	Weak acid
6	1 M CH₃COOH	Weak acid
7	Microwave (700 W/10 min)	Heat/radiation
8	1% (v/v) Triton X-100	Detergent
9	1% (v/v) Tween-80	Detergent
10	50 mM DTT	Detergent
11	Autoclaved (121 °C/20 min)	Heat/pressure
12	Demi water + demi water	Negative control
13	Demi water + TMEs	Negative control
14	1 M NaOH + demi water	Negative control
15	1 M HCl + demi water	Negative control
16	Autoclaved (121 °C/20 min) + demi water	Negative control

A volume of 3 mL of 30% (v/v) fat Schwanniomyces occidentalis cells was submitted to 10 mL of a chemical listed below. For the microwave, autoclave sterilization, and negative control, 10 mL of demi water was used. Pretreatments were performed for 24 h unless stated otherwise

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