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Table 2 Overview of the different pretreatment procedures taken in this study

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Sample number Method/added chemical Effect
1 1 M NaCl Osmotic pressure
2 1 M NaOH Strong base
3 1 M NH4OH Weak base
4 1 M HCl Strong acid
5 1 M H3PO4 Weak acid
6 1 M CH3COOH Weak acid
7 Microwave (700 W/10 min) Heat/radiation
8 1% (v/v) Triton X-100 Detergent
9 1% (v/v) Tween-80 Detergent
10 50 mM DTT Detergent
11 Autoclaved (121 °C/20 min) Heat/pressure
12 Demi water + demi water Negative control
13 Demi water + TMEs Negative control
14 1 M NaOH + demi water Negative control
15 1 M HCl + demi water Negative control
16 Autoclaved (121 °C/20 min) + demi water Negative control
  1. A volume of 3 mL of 30% (v/v) fat Schwanniomyces occidentalis cells was submitted to 10 mL of a chemical listed below. For the microwave, autoclave sterilization, and negative control, 10 mL of demi water was used. Pretreatments were performed for 24 h unless stated otherwise