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Table 1 Parameters used for the bioreactor cultivations of Schwanniomyces occidentalis and Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Parameter

S. occidentalis

T. harzianum

Agitation (rpm)

300–1200

300–1200

Dissolved oxygen control (%)

20—agitation cascade controlled

20—agitation cascade controlled

Temperature (°C)

30

30

pH

5.5 ± 0.1

4.9 ± 0.1

Acid

6 M H3PO4—pump speed 20%

6 M H3PO4—pump speed 20%

Base

6 M NaOH—pump speed 20%

6 M NaOH—pump speed 20%

Feed

YPD

S. occidentalis—pump speed 10%

Gasflow (vvm)

0.5—pressurized air

0.5—pressurized air

Cultivation duration batch phase

18–22 h—until DO and pH started rising

36–40 h—until DO and pH started rising

Cultivation duration fed-batch phase

160–180 h total cultivation time

~ 140–180 h total cultivation time