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Table 1 Parameters used for the bioreactor cultivations of Schwanniomyces occidentalis and Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

ParameterS. occidentalisT. harzianum
Agitation (rpm)300–1200300–1200
Dissolved oxygen control (%)20—agitation cascade controlled20—agitation cascade controlled
Temperature (°C)3030
pH5.5 ± 0.14.9 ± 0.1
Acid6 M H3PO4—pump speed 20%6 M H3PO4—pump speed 20%
Base6 M NaOH—pump speed 20%6 M NaOH—pump speed 20%
FeedYPDS. occidentalis—pump speed 10%
Gasflow (vvm)0.5—pressurized air0.5—pressurized air
Cultivation duration batch phase18–22 h—until DO and pH started rising36–40 h—until DO and pH started rising
Cultivation duration fed-batch phase160–180 h total cultivation time~ 140–180 h total cultivation time