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Table 1 Parameters used for the bioreactor cultivations of Schwanniomyces occidentalis and Trichoderma harzianum

From: Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Parameter S. occidentalis T. harzianum
Agitation (rpm) 300–1200 300–1200
Dissolved oxygen control (%) 20—agitation cascade controlled 20—agitation cascade controlled
Temperature (°C) 30 30
pH 5.5 ± 0.1 4.9 ± 0.1
Acid 6 M H3PO4—pump speed 20% 6 M H3PO4—pump speed 20%
Base 6 M NaOH—pump speed 20% 6 M NaOH—pump speed 20%
Feed YPD S. occidentalis—pump speed 10%
Gasflow (vvm) 0.5—pressurized air 0.5—pressurized air
Cultivation duration batch phase 18–22 h—until DO and pH started rising 36–40 h—until DO and pH started rising
Cultivation duration fed-batch phase 160–180 h total cultivation time ~ 140–180 h total cultivation time