Fig. 3

Pretreatment screening analysis. The pretreatment screening analysis exposed the Schwanniomyces occidentalis fat cells to chemicals, microwave or autoclaving pretreatment for 24 h prior to addition of the tailor-made enzymes (TMEs). The release of oil was visualized after 24 h of TME incubation time by the addition of Nile red. Sample (1) 1 M NaCl, (2) 1 M NaOH, (3) 1 M NH₄OH, (4) 1 M HCl, (5) 1 M H₃PO₄, (6) 1 M CH₃COOH, (7) microwave procedure (700 W, 10 min), (8) 1% (v/v) Triton X-100, (9) 1% (v/v) Tween-80, (10) 50 mM DTT, (11) autoclave procedure (121 °C/20 min), (12) control sample with demi water as a pretreatment step and demi water as a lysis procedure, (13) control sample with demi water as a pretreatment step and lytic enzymes as a lysis procedure, (14) control sample with 1 M NaOH as pretreatment step and demi water as a lysis procedure, (15) control sample with HCl as a pretreatment step and demi water as a lysis procedure, (16) control sample by autoclave procedure (121 °C/20 min) and demi water as a lysis procedure