Fig. 3

Applying the HpFMD promoter for expression of the enzymes HRP (a), CalB (b) and MeHNL (c) confirms the high expression observed with eGFP (Fig. 1). The strains were grown in DWPs on BMD1 media until glucose depletion for 60 h and were subsequently induced with methanol. HRP and CalB activities in the supernatants were measured and cells lysed to measure intracellular MeHNL activity. Mean values (MVs) and standard deviation (SDs) of biological quadruplicates are shown. The activities of the methanol induced HpFMD promoter compared to the state of the art AOX1 promoter are highlighted (after 72 h of methanol induction)