Fig. 2From: Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastorisPHpFMD enables strong derepression and exceeds the strength of methanol induced endogenous P. pastoris promoters. Strains bearing selected promoters from Fig. 1 (HpFMD, HpMOX, PpAOX1, PpCAT1, PpGAP) were cultivated in shake flasks and inoculated to a low starting OD600 of 0.05. Reporter protein fluorescence, OD600 and glucose levels were measured. Fluorescence/OD600 values at t = 0 are not shown, as the starting OD600 of 0.05 was outside the linear range of the spectrometer used. The initial glucose concentration of the media was 55.5 mM (10 g/L). MVs and SDs of biological triplicates are shownBack to article page