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Table 2 Effect of environmental factors on co-production of various enzymes by A. niger APS

From: A novel multi-enzyme preparation produced from Aspergillus niger using biodegradable waste: a possible option to combat heterogeneous biofilms

Factor CMCase FPase β-Glucosidase Pectinase Mannanase Xylanase Gluco-amylase α-Amylase Alginate lyase
Enzyme activity (IU/ml)
Incubation temperature (°C)
 20 1.44 ± 0.017 0.0 0.17 ± 0.04 4.5 ± 0.98 0.70 ± 0.003 11.33 ± 1.78 0.0 614.9 ± 5.58 0.0
 25 2.45 ± 0.014 0.86 ± 0.024 0.84 ± 0.038 6.55 ± 0.106 2.08 ± 0.014 28.48 ± 0.31 18.42 ± 2.04 2392.3 ± 21.68 1.23 ± 0.04
 30 3.84 ± 0.012 1.21 ± 0.04 3.62 ± 0.028 14.55 ± 0.15 7.33 ± 1.07 39.48 ± 1.32 42.31 ± 2.56 2774 ± 2.67 3.32 ± 0.016
 35 2.26 ± 0.012 0.49 ± 0.026 1.95 ± 0.011 10.75 ± 0.304 3.04 ± 0.028 41.14 ± 1.04 41.87 ± 3.84 2073 ± 18.9 2.72 ± 0.016
 40 2.37 ± 0.014 0.35 ± 0.06 1.85 ± 0.078 5.96 ± 0.098 2.39 ± 0.018 30.39 ± 1.86 36.42 ± 2.03 1182 ± 12.32 1.87 ± 0.011
 50 0.37 ± 0.002 0.0 0.0 0.0 0.47 ± 0.01 0.0 0.0 0.0 0.0
Initial pH
  2.0 0.27 ± 0.06 0.31 ± 0.02 0.0 4.87 ± 0.068 0.38 ± 0.04 23.12 ± 2.14 0.0 433.3 ± 6.45 0.0
  3.0 2.67 ± 0.014 0.53 ± 0.042 2.51 ± 0.012 8.35 ± 0.22 6.30 ± 1.08 31.04 ± 2.86 37.11 ± 3.14 2005.5 ± 23.25 1.26 ± 0.011
  4.0 3.85 ± 0.014 1.24 ± 0.04 3.72 ± 0.20 13.92 ± 0.18 7.58 ± 0.98 41.58 ± 1.12 39.10 ± 4.48 2759.6 ± 30.62 3.26 ± 0.032
  5.0 3.13 ± 0.012 1.02 ± 0.023 3.40 ± 0.012 14.26 ± 0.12 6.67 ± 0.23 41.82 ± 1.56 37.55 ± 2.28 2629.7 ± 35.56 3.53 ± 0.028
  6.0 2.44 ± 0.014 0.98 ± 0.012 2.63 ± 0.025 13.34 ± 0.046 4.28 ± 0.067 42.05 ± 0.067 36.31 ± 1.13 2428.9 ± 27.45 4.48 ± 0.06
  7.0 2.51 ± 0.018 0.92 ± 0.01 1.66 ± 0.015 6.14 ± 0.86 4.46 ± 0.42 48.41 ± 2.13 11.72 ± 1.86 2302.3 ± 20.45 4.73 ± 0.07
  8.0 2.23 ± 0.014 0.89 ± 0.06 1.69 ± 0.011 5.67 ± 0.042 4.26 ± 0.045 48.02 ± 1.12 10.86 ± 1.12 2236.7 ± 24.37 3.52 ± 0.011
  9.0 2.17 ± 0.016 0.77 ± 0.03 1.39 ± 0.012 5.37 ± 0.37 3.52 ± 0.014 44.84 ± 3.86 9.87 ± 0.09 2214.7 ± 36.78 1.72 ± 0.017
  10.0 2.17 ± 0.011 0.73 ± 0.03 0.83 ± 0.011 4.51 ± 0.021 3.12 ± 0.012 42.43 ± 1.07 2.59 ± 0.15 2104.6 ± 27.42 1.12 ± 0.06