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Fig. 1 | AMB Express

Fig. 1

From: A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli)

Fig. 1

Images displaying the PCR amplicons of proinsulin DNA, purified vector DNA, and the integrated pET21b-hPin vector with the inserted proinsulin gene. Lanes 1 and 12 contain one kb molecular weight marker. Lanes 2 to 7 contains the PCR amplicons of proinsulin flanked with BamHI and XhoI restricted ends; Lane 8 contains the purified pET21b miniprep product; Lanes 9 to 11 contain the integrated pET21b-hPin vector miniprep products

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