Fig. 1From: A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli)Images displaying the PCR amplicons of proinsulin DNA, purified vector DNA, and the integrated pET21b-hPin vector with the inserted proinsulin gene. Lanes 1 and 12 contain one kb molecular weight marker. Lanes 2 to 7 contains the PCR amplicons of proinsulin flanked with BamHI and XhoI restricted ends; Lane 8 contains the purified pET21b miniprep product; Lanes 9 to 11 contain the integrated pET21b-hPin vector miniprep productsBack to article page