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AMB Express

Table 1 Primers used in this study

From: A safety type of genetically engineered bacterium that degrades chemical pesticides

No

Restriction enzymesa

Sequence (5′ → 3′)b

1

EcoRI

GGGAATTCATGGTGAGCAAGGGCGAGGAGCT

2

BamHI

TTGGATCCCTTGTACAGCTCGTCCATGCCGAG

3

SacI

TTCGAGCTCCGTGCGTGTTGACTATTTTACCT

4

XbaI

GCTCTAGATCGGCAAGGTGTTCTGGTC

5

BglII

GAAGATCTATGAGTTTGGAAAGCTTAACCGT

6

PstI

AACTGCAGTCAAAACAGCTCATCATTCACGTACAT

  1. aRestriction enzyme that can digest the sequences underlined
  2. bUnderlined sequences are restriction sites
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