Fig. 5

In vitro biofilm inhibition assay. S. epidermidis was grown under microaerobic conditions. a Different metabolic inhibitors were added as indicated: 100 µM NaCN, 1 mM B1,4BP or both inhibitors. b Different concentrations of the uncoupler CCCP (0.5, 1.0 and 1.5 µM) were added to deplete ATP. After 24 h of incubation biofilm generation was evaluated by measuring the absorbance at 492 nm with a microplate reader. Each sample was compared with the control (without additions). Statistics were applied using ANOVA and Dunnett’s post hoc test. Significance *P < 0.0001