Fig. 2
From: A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities
The antibacterial activity of the elution gradient of silica gel column chromatography. Antimicrobial activity of five fractions of ethyl acetate phase of GN59 fermentation broth the number 1, 2, 3, 4, 5 represents different fractions eluted by eluents (petroleum ether:ethyl acetate 100:0; petroleum ether:ethyl acetate 75:25; petroleum ether:ethyl acetate 50:50; petroleum ether:ethyl acetate 25:75; petroleum ether:ethyl acetate 0:100). 6 and 7 represent fractions eluted by ethyl acetate: methanol 75:25. 8 and 9 represent fractions eluted by ethyl acetate: methanol 50:50; 10 and 11 represent fractions eluted by ethyl acetate: methanol 25:75. 12 and 13 represent fractions eluted by ethyl acetate: methanol 0:100. The No. 10 and 11 have antibacterial activity agaist E.coli. The experiment was repeated three times