Skip to main content

Advertisement

Table 1 Optimal dilutions for antibody and coating antigen for VLP-based I-ELISA

From: Self-assembly into virus–like particles of the recombinant capsid protein of porcine circovirus type 3 and its application on antibodies detection

Serum dilutionConcentration of coating antigen (X ± SD, µg/ml)
10.07.55.02.51.00.5
50 × (+)2.646 ± 0.0162.338 ± 0.0061.645 ± 0.0231.46 ± 0.0591.1 ± 0.0220.838 ± 0.019
50 × (−)0.305 ± 0.0140.3 ± 0.0870.234 ± 0.1120.174 ± 0.0060.132 ± 0.0150.112 ± 0.069
P/N8.6757.7937.0308.3918.3337.482
100 × (+)2.088 ± 0.0031.797 ± 0.0151.552 ± 0.0291.104 ± 0.0840.832 ± 0.0480.669 ± 0.001
100 × (−)0.181 ± 0.0750.166 ± 0.0060.147 ± 0.0980.12 ± 0.0690.095 ± 0.0030.085 ± 0.004
P/N11.53610.82510.5589.2008.7587.871
150 × (+)1.72 ± 0.0671.524 ± 0.0361.339 ± 0.0520.962 ± 0.0260.756 ± 0.0360.576 ± 0.006
150 × (−)0.155 ± 0.0570.132 ± 0.0190.122 ± 0.0220.094 ± 0.0680.082 ± 0.0470.077 ± 0.024
P/N11.09711.54510.97510.2349.2207.481
200 × (+)1.604 ± 0.0061.376 ± 0.0171.338 ± 0.0111.079 ± 0.0470.777 ± 0.0330.584 ± 0.005
200 × (−)0.126 ± 0.0440.109 ± 0.0250.102 ± 0.0970.097 ± 0.0140.081 ± 0.0740.093 ± 0.016
P/N12.73012.62413.11811.1249.5936.280