Self-assembly into virus–like particles of the recombinant capsid protein of porcine circovirus type 3 and its application on antibodies detection

AMB Express

Table 1 Optimal dilutions for antibody and coating antigen for VLP-based I-ELISA

Serum dilution	Concentration of coating antigen (X ± SD, µg/ml)
Serum dilution	10.0	7.5	5.0	2.5	1.0	0.5
50 × (+)	2.646 ± 0.016	2.338 ± 0.006	1.645 ± 0.023	1.46 ± 0.059	1.1 ± 0.022	0.838 ± 0.019
50 × (−)	0.305 ± 0.014	0.3 ± 0.087	0.234 ± 0.112	0.174 ± 0.006	0.132 ± 0.015	0.112 ± 0.069
P/N	8.675	7.793	7.030	8.391	8.333	7.482
100 × (+)	2.088 ± 0.003	1.797 ± 0.015	1.552 ± 0.029	1.104 ± 0.084	0.832 ± 0.048	0.669 ± 0.001
100 × (−)	0.181 ± 0.075	0.166 ± 0.006	0.147 ± 0.098	0.12 ± 0.069	0.095 ± 0.003	0.085 ± 0.004
P/N	11.536	10.825	10.558	9.200	8.758	7.871
150 × (+)	1.72 ± 0.067	1.524 ± 0.036	1.339 ± 0.052	0.962 ± 0.026	0.756 ± 0.036	0.576 ± 0.006
150 × (−)	0.155 ± 0.057	0.132 ± 0.019	0.122 ± 0.022	0.094 ± 0.068	0.082 ± 0.047	0.077 ± 0.024
P/N	11.097	11.545	10.975	10.234	9.220	7.481
200 × (+)	1.604 ± 0.006	1.376 ± 0.017	1.338 ± 0.011	1.079 ± 0.047	0.777 ± 0.033	0.584 ± 0.005
200 × (−)	0.126 ± 0.044	0.109 ± 0.025	0.102 ± 0.097	0.097 ± 0.014	0.081 ± 0.074	0.093 ± 0.016
P/N	12.730	12.624	13.118	11.124	9.593	6.280