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Table 2 Effect of chemical reagents on hydrolytic activity of BsKER71

From: Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4

Surfactant

Casein as substrate

Keratin as substrate

1 mmol/L

5 m mol/L

1 mmol/L

5 mmol/L

CK

100.00

100.00

100.00

100.00

SDS

103.74

97.12

86.28

0.00

DTT

103.74

96.92

100.87

91.55

Triton-100

107.72

98.37

80.07

103.66

Na2SO3

93.63

98.99

101.38

0.00

DMSO

107.53

16.17

103.70

0.00