Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4

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Table 1 Effect of various metal ions on hydrolytic activity of BsKER71

Metal ions	Concentration (mmol/L)	Hydrolytic activity of different substrate
Metal ions	Concentration (mmol/L)	Casein (%)	Keratin (%)
CK	0	100.00	100.00
Zn²⁺	2	97.00	95.48
Co²⁺	2	93.73	92.70
Mg²⁺	2	114.73	112.13
Ca²⁺	2	116.64	115.81
Mn²⁺	2	94.71	89.45
Ni²⁺	2	95.36	94.21
Cu²⁺	2	2.78	1.14