Fig. 1
From: Production of foot-and-mouth disease virus SAT2 VP1 protein
Expression of the VP1 gene of the FMDV SAT2/ZIM/7/83 in E. coli BL21 (DE3) cells. The 615-bp VP1 gene was PCR amplified and electrophoresed on a 1.5% agarose gel (a). The GeneRulerTM 1 kb DNA Ladder (Thermo Scientific) was loaded as a marker (M). The recombinant VP1 protein was produced by EC-VP1 after induction with a final concentration of 1 mM IPTG in EnPresso B medium (b). After cell lysis with BPER, the insoluble fraction was analyzed on a 15% SDS–polyacrylamide gel, the expressed VP1 protein is indicated with an arrow. The PageRulerTM Prestained Protein Ladder (Thermo Fischer Scientific) was included as a marker in lanes M in b, c. The N-terminal histidine tagged VP1 protein was detected with anti-histidine antibodies by western blot analysis among insoluble proteins that were separated under denaturing conditions and transferred to a PVDF membrane (c)