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Table 4 Analysis of variance (ANOVA) of the influence of temperature, time and IPTG concentration on biomass and ΔNS1 solubility

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Source of variation

Biomass (DCW g/L)a

ΔNS1 (mg/L)b

Sum of squares

df

Mean square

F-calc

p-value

Sum of squares

df

Mean square

F-calc

p-value

Regression

82.49

9

9.17

34.83

< 0.0001

2.5 × 106

9

2.8 × 105

19.52

< 0.0001

Residuals

2.63

10

0.26

  

1.4 × 105

10

14,251

  

Lack of fit

1.83

5

0.37

2.30

0.1913

4.2 × 104

5

8335

0.31

0.8228

Pure error Total

0.80

5

0.16

  

1.0 × 105

5

20,168

  
  1. aModel: 7.35 + 0.88*B − 1.20*AB − 1.86*A2 − 0.83*B2 − 0.78*C2, R2 = 0.97; Adj. R2 = 0.95; Pred. R2 = 0.92. Adeq. precision = 21.9. F-tab = 2.96
  2. bModel: 969 − 79*A − 134*AB − 291*A2 − 238*B2 − 215*C2, R2 = 0.94; Adj. R2 = 0.92; Pred. R2 = 0.90. Adeq. precision = 16.9. F-tab = 2.96
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