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Table 4 Analysis of variance (ANOVA) of the influence of temperature, time and IPTG concentration on biomass and ΔNS1 solubility

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Source of variationBiomass (DCW g/L)aΔNS1 (mg/L)b
Sum of squaresdfMean squareF-calcp-valueSum of squaresdfMean squareF-calcp-value
Regression82.4999.1734.83< 0.00012.5 × 10692.8 × 10519.52< 0.0001
Residuals2.63100.26  1.4 × 1051014,251  
Lack of fit1.8350.372.300.19134.2 × 104583350.310.8228
Pure error Total0.8050.16  1.0 × 105520,168  
  1. aModel: 7.35 + 0.88*B − 1.20*AB − 1.86*A2 − 0.83*B2 − 0.78*C2, R2 = 0.97; Adj. R2 = 0.95; Pred. R2 = 0.92. Adeq. precision = 21.9. F-tab = 2.96
  2. bModel: 969 − 79*A − 134*AB − 291*A2 − 238*B2 − 215*C2, R2 = 0.94; Adj. R2 = 0.92; Pred. R2 = 0.90. Adeq. precision = 16.9. F-tab = 2.96