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Table 3 Estimated effects for IPTG concentration, temperature and time of induction on biomass and ΔNS1 solubility

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

FactorsBiomass (DCW g/L)ΔNS1 (mg/L)
EffectStandard errorp-valueEffectStandard errorp-value
Mean7.350.21< 0.000196949< 0.0001
A: Temp− 0.120.140.4032− 79320.0352
B: Time0.880.14< 0.000110320.7601
C: IPTG0.000.140.997516320.6377
AB− 1.200.18< 0.0001− 134420.0100
AC− 0.060.180.748620420.6461
BC0.130.180.48510420.9915
A2− 1.860.14< 0.0001− 29131< 0.0001
B2− 0.830.14< 0.0001− 23831< 0.0001
C2− 0.780.140.0002− 21531< 0.0001