AMB Express

Table 3 Estimated effects for IPTG concentration, temperature and time of induction on biomass and ΔNS1 solubility

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Factors	Biomass (DCW g/L)			ΔNS1 (mg/L)
Factors	Effect	Standard error	p-value	Effect	Standard error	p-value
Mean	7.35	0.21	< 0.0001	969	49	< 0.0001
A: Temp	− 0.12	0.14	0.4032	− 79	32	0.0352
B: Time	0.88	0.14	< 0.0001	10	32	0.7601
C: IPTG	0.00	0.14	0.9975	16	32	0.6377
AB	− 1.20	0.18	< 0.0001	− 134	42	0.0100
AC	− 0.06	0.18	0.7486	20	42	0.6461
BC	0.13	0.18	0.4851	0	42	0.9915
A²	− 1.86	0.14	< 0.0001	− 291	31	< 0.0001
B²	− 0.83	0.14	< 0.0001	− 238	31	< 0.0001
C²	− 0.78	0.14	0.0002	− 215	31	< 0.0001

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